• Western blot analysis of various lysates, using Septin 1 antibody (STJ119580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of Rat spleen, using Septin 1 antibody (STJ119580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using Septin 1 antibody (STJ119580) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using Septin 1 antibody (STJ119580) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Septin 1 antibody (STJ119580) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SEPT1 antibody (270-370) (STJ119580)

SKU:
STJ119580

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEPT1 (270-370) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEPTIN1
Gene ID: 1731
Uniprot ID: SEPT1_HUMAN
Immunogen Region: 270-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-370 of human Septin 1 (NP_443070.5).
Immunogen Sequence: IPFAVVGSCEVVRDGGNRPV RGRRYSWGTVEVENPHHCDF LNLRRMLVQTHLQDLKEVTH DLLYEGYRARCLQSLARPGA RDRASRSKLSRQSATEIPLP M
Tissue Specificity Expressed at high levels in lymphoid and hematopoietic tissues.
Function Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential).
Protein Name Septin-1
Larp
Peanut-Like Protein 3
Serologically Defined Breast Cancer Antigen Ny-Br-24
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Remains At The Centrosomes And The Nearby Microtubules Throughout Mitosis
Localizes To The Midbody During Cytokinesis
Alternative Antibody Names Anti-Septin-1 antibody
Anti-Larp antibody
Anti-Peanut-Like Protein 3 antibody
Anti-Serologically Defined Breast Cancer Antigen Ny-Br-24 antibody
Anti-SEPTIN1 antibody
Anti-DIFF6 antibody
Anti-PNUTL3 antibody
Anti-SEPT1 antibody

Information sourced from Uniprot.org

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