• Immunofluorescence analysis of MCF7 cells using SEPHS1 antibody (STJ115365). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from wild type (WT) and SEPHS1 knockout (KO) 293T (KO) cells, using SEPHS1 antibody (STJ115365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SEPHS1 antibody (1-392) (STJ115365)

SKU:
STJ115365

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEPHS1 (1-392) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEPHS1
Gene ID: 22929
Uniprot ID: SPS1_HUMAN
Immunogen Region: 1-392
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2).
Immunogen Sequence: MSTRESFNPESYELDKSFRL TRFTELKGTGCKVPQDVLQK LLESLQENHFQEDEQFLGAV MPRLGIGMDTCVIPLRHGGL SLVQTTDYIYPIVDDPYMMG RIACANVLSDLYAMGVTECD NMLMLLGVSNKMTDRERDKV MPLIIQGFKDAAEEAGTSVT GGQTVLNPWIVLGGVATTVC QPNEFIMPDNAVPGDVLVLT KPLGTQVAVAVHQWLDIPEK WNKIKLVVTQEDVELAYQE
Tissue Specificity Isoform 1: Gradually expressed during the cell cycle until G2/M phase and then decreases. Isoform 2: Gradually expressed during the cell cycle until G2/M phase and then decreases. Isoform 3: Gradually expressed during the cell cycle until S phase and then decreases.
Function Synthesizes selenophosphate from selenide and ATP.
Protein Name Selenide - Water Dikinase 1
Selenium Donor Protein 1
Selenophosphate Synthase 1
Cellular Localisation Isoform 1: Cell Membrane
Peripheral Membrane Protein
Nucleus Membrane
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-Selenide - Water Dikinase 1 antibody
Anti-Selenium Donor Protein 1 antibody
Anti-Selenophosphate Synthase 1 antibody
Anti-SEPHS1 antibody
Anti-SELD antibody
Anti-SPS antibody
Anti-SPS1 antibody

Information sourced from Uniprot.org

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