• Immunofluorescence analysis of L929 cells using SEMA3C Rabbit polyclonal antibody (STJ117581) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Human serum, using SEMA3C Rabbit polyclonal antibody (STJ117581) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SEMA3C antibody (21-330) (STJ117581)

SKU:
STJ117581

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEMA3C (21-330) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEMA3C
Gene ID: 10512
Uniprot ID: SEM3C_HUMAN
Immunogen Region: 21-330
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-330 of human SEMA3C (NP_006370.1).
Immunogen Sequence: GSSQPQARVYLTFDELRETK TSEYFSLSHHPLDYRILLMD EDQDRIYVGSKDHILSLNIN NISQEALSVFWPASTIKVEE CKMAGKDPTHGCGNFVRVIQ TFNRTHLYVCGSGAFSPVCT YLNRGRRSEDQVFMIDSKCE SGKGRCSFNPNVNTVSVMIN EELFSGMYIDFMGTDAAIFR SLTKRNAVRTDQHNSKWLSE PMFVDAHVIPDGTDPNDAKV YFFFKEKLTDNNRSTKQIH
Tissue Specificity Expressed intensely in the heart, skeletal muscle, colon, small intestine, ovary, testis, and prostate. Faint expression ubiquitously among other organs, including brain.
Function Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance.
Protein Name Semaphorin-3c
Semaphorin-E
Sema E
Cellular Localisation Secreted
Alternative Antibody Names Anti-Semaphorin-3c antibody
Anti-Semaphorin-E antibody
Anti-Sema E antibody
Anti-SEMA3C antibody
Anti-SEMAE antibody

Information sourced from Uniprot.org

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