Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4ยฐC overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-SELE antibody (100-150 aa) (STJ98576)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human SELE at the amino acid range 100-150
STJ98576
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General Information

Short DescriptionRabbit polyclonal anti-E-selectin (100-150 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC/IF/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeIHC-P 100-300
WB 1:500-2000
ELISA 1:10000-20000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSELE
Gene ID6401
Uniprot IDLYAM2_HUMAN
ImmunogenThe antiserum was produced against synthesized peptide derived from the N-terminal region of human SELE at the amino acid range 100-150
Immunogen Region100-150 aa
SpecificityE-Selectin Polyclonal Antibody detects endogenous levels of E-Selectin

Additional Info

Protein Name E-Selectin
Cd62 Antigen-Like Family Member E
Endothelial Leukocyte Adhesion Molecule 1
Elam-1
Leukocyte-Endothelial Cell Adhesion Molecule 2
Lecam2
Cd Antigen Cd62e
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-E-Selectin antibody
Anti-Cd62 Antigen-Like Family Member E antibody
Anti-Endothelial Leukocyte Adhesion Molecule 1 antibody
Anti-Elam-1 antibody
Anti-Leukocyte-Endothelial Cell Adhesion Molecule 2 antibody
Anti-Lecam2 antibody
Anti-Cd Antigen Cd62e antibody
Anti-SELE antibody
Anti-ELAM1 antibody

Information sourced from Uniprot.org

Citations

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