Anti-SECISBP2 antibody (STJA0006894)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human SECISBP2
General Information
| Short Description | Rabbit polyclonal anti-Selenocysteine insertion sequence-binding protein 2 for use in WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SECISBP2 |
| Gene ID | 79048 |
| Uniprot ID | SEBP2_HUMAN |
| Immunogen | Synthesized peptide derived from human SECISBP2 |
| Specificity | This antibody detects endogenous levels of SECISBP2 at Human, Rat |
Additional Info
| Function | mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. |
| Protein Name | Selenocysteine Insertion Sequence-Binding Protein 2Secis-Binding Protein 2 |
| Database Links | Reactome: R-HSA-2408557 |
| Cellular Localisation | Isoform 1: NucleusIsoform 2: Mitochondrion |
| Alternative Antibody Names | Anti-Selenocysteine Insertion Sequence-Binding Protein 2 antibodyAnti-Secis-Binding Protein 2 antibodyAnti-SECISBP2 antibodyAnti-SBP2 antibody |
Information sourced from Uniprot.org