• Western blot analysis of various lysates using SEC61A1 Rabbit polyclonal antibody (STJ113260) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SEC61A1 antibody (310-420) (STJ113260)

SKU:
STJ113260

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEC61A1 (310-420) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEC61A1
Gene ID: 29927
Uniprot ID: S61A1_HUMAN
Immunogen Region: 310-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human SEC61A1 (NP_037468.1).
Immunogen Sequence: ARFSGNLLVSLLGTWSDTSS GGPARAYPVGGLCYYLSPPE SFGSVLEDPVHAVVYIVFML GSCAFFSKTWIEVSGSSAKD VAKQLKEQQMVMRGHRETSM VHELNRYIPTA
Tissue Specificity Expressed in proximal and distal tubules in kidney (at protein level).
Function Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER. Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis. Plays a critical role in nephrogenesis, specifically at pronephros stage.
Protein Name Protein Transport Protein Sec61 Subunit Alpha Isoform 1
Sec61 Alpha-1
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-1799339
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Localizes Exclusively In Granular Structures In The Endoplasmic Reticulum (Er)
Alternative Antibody Names Anti-Protein Transport Protein Sec61 Subunit Alpha Isoform 1 antibody
Anti-Sec61 Alpha-1 antibody
Anti-SEC61A1 antibody
Anti-SEC61A antibody

Information sourced from Uniprot.org

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