| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SCP2 (1-322) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SCP2 |
| Gene ID: |
6342 |
| Uniprot ID: |
SCP2_HUMAN |
| Immunogen Region: |
1-322 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human SCP2 (NP_001007099.1). |
| Immunogen Sequence: |
MSSSPWEPATLRRVFVVGVG MTKFVKPGAENSRDYPDLAE EAGKKALADAQIPYSAVDQA CVGYVFGVAECVLALGFEKM SKGSLGIKFSDRTIPTDKHV DLLINKYGLSAHPVAPQMFG YAGKEHMEKYGTKIEHFAKI GWKNHKHSVNNPYSQFQDEY SLDEVMASKEVFDFLTILQC CPTSDGAAAAILASEAFVQK YGLQSKAVEILAQEMMTDLP SSFEEKSIIKMVGFDMSKE |
| Tissue Specificity | Liver, fibroblasts, and placenta. |
| Post Translational Modifications | Isoform SCP2: preSCP2, a protein with a molecular mass of about 15 kDa, is processed into its mature form (SCP2) by proteolytic cleavage of a 20 residue leader sequence after translocation into peroxisomes. |
| Function | Isoform SCPx: Plays a crucial role in the peroxisomal oxidation of branched-chain fatty acids. Catalyzes the last step of the peroxisomal beta-oxidation of branched chain fatty acids and the side chain of the bile acid intermediates di- and trihydroxycoprostanic acids (DHCA and THCA). Also active with medium and long straight chain 3-oxoacyl-CoAs. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol and transfers phosphatidylcholine and 7-dehydrocholesterol between membrances, in vitro. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs. Isoform SCP2: Mediates the transfer of all common phospholipids, cholesterol and gangliosides from the endoplasmic reticulum to the plasma membrane. May play a role in regulating steroidogenesis. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol. Also binds fatty acids and fatty acyl Coenzyme A (CoA) such as phytanoyl-CoA. Involved in the regulation phospholipid synthesis in endoplasmic reticulum enhancing the incorporation of exogenous fatty acid into glycerides. Seems to stimulate the rate-limiting step in phosphatidic acid formation mediated by GPAT3. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs. |
| Protein Name | Sterol Carrier Protein 2Scp-2Acetyl-Coa C-MyristoyltransferaseNon-Specific Lipid-Transfer ProteinNsl-TpPropanoyl-Coa C-AcyltransferaseScp-2/3-Oxoacyl-Coa ThiolaseScp-2/ThiolaseScp-ChiScpxSterol Carrier Protein XScp-X |
| Database Links | Reactome: R-HSA-193368 P22307-1Reactome: R-HSA-2046106Reactome: R-HSA-389887 P22307-1Reactome: R-HSA-9033241Reactome: R-HSA-9033500 |
| Cellular Localisation | Isoform Scp2: PeroxisomeCytoplasmMitochondrionEndoplasmic ReticulumIsoform Scpx: Peroxisome |
| Alternative Antibody Names | Anti-Sterol Carrier Protein 2 antibodyAnti-Scp-2 antibodyAnti-Acetyl-Coa C-Myristoyltransferase antibodyAnti-Non-Specific Lipid-Transfer Protein antibodyAnti-Nsl-Tp antibodyAnti-Propanoyl-Coa C-Acyltransferase antibodyAnti-Scp-2/3-Oxoacyl-Coa Thiolase antibodyAnti-Scp-2/Thiolase antibodyAnti-Scp-Chi antibodyAnti-Scpx antibodyAnti-Sterol Carrier Protein X antibodyAnti-Scp-X antibodyAnti-SCP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SPK knockout (KO) 293T cells, using [KO Validated] SPK Rabbit polyclonal antibody (STJ11100823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89759/358235/STJ11100823_1__81500.1713122567.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SPK knockout (KO) 293T cells, using [KO Validated] SPK Rabbit polyclonal antibody (STJ11100823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
