• Western blot analysis of extracts of mouse liver, using SCP2 Antibody (STJ27335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human liver cancer using SCP2 antibody (STJ27335) at dilution of 1:200 (40x lens).

Anti-SCP2 antibody (1-322) (STJ27335)

SKU:
STJ27335

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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-SCP2 (1-322) is suitable for use in Western Blot and Immunohistochemistry.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: SCP2
Gene ID: 6342
Uniprot ID: SCP2_HUMAN
Immunogen Region: 1-322
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human SCP2 (NP_001007099.1).
Tissue Specificity Liver, fibroblasts, and placenta.
Post Translational Modifications Isoform SCP2: preSCP2, a protein with a molecular mass of about 15 kDa, is processed into its mature form (SCP2) by proteolytic cleavage of a 20 residue leader sequence after translocation into peroxisomes.
Function Isoform SCPx: Plays a crucial role in the peroxisomal oxidation of branched-chain fatty acids. Catalyzes the last step of the peroxisomal beta-oxidation of branched chain fatty acids and the side chain of the bile acid intermediates di- and trihydroxycoprostanic acids (DHCA and THCA). Also active with medium and long straight chain 3-oxoacyl-CoAs. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol and transfers phosphatidylcholine and 7-dehydrocholesterol between membrances, in vitro. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs. Isoform SCP2: Mediates the transfer of all common phospholipids, cholesterol and gangliosides from the endoplasmic reticulum to the plasma membrane. May play a role in regulating steroidogenesis. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol. Also binds fatty acids and fatty acyl Coenzyme A (CoA) such as phytanoyl-CoA. Involved in the regulation phospholipid synthesis in endoplasmic reticulum enhancing the incorporation of exogenous fatty acid into glycerides. Seems to stimulate the rate-limiting step in phosphatidic acid formation mediated by GPAT3. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs.
Protein Name Sterol Carrier Protein 2
Scp-2
Acetyl-Coa C-Myristoyltransferase
Non-Specific Lipid-Transfer Protein
Nsl-Tp
Propanoyl-Coa C-Acyltransferase
Scp-2/3-Oxoacyl-Coa Thiolase
Scp-2/Thiolase
Scp-Chi
Scpx
Sterol Carrier Protein X
Scp-X
Straight-Chain Acyl-Coa Oxidase
Scox
Database Links Reactome: R-HSA-193368 P22307-1
Reactome: R-HSA-2046106
Reactome: R-HSA-389887 P22307-1
Reactome: R-HSA-9033241
Reactome: R-HSA-9033500
Cellular Localisation Isoform Scp2: Peroxisome
Cytoplasm
Mitochondrion
Endoplasmic Reticulum
Isoform Scpx: Peroxisome
Alternative Antibody Names Anti-Sterol Carrier Protein 2 antibody
Anti-Scp-2 antibody
Anti-Acetyl-Coa C-Myristoyltransferase antibody
Anti-Non-Specific Lipid-Transfer Protein antibody
Anti-Nsl-Tp antibody
Anti-Propanoyl-Coa C-Acyltransferase antibody
Anti-Scp-2/3-Oxoacyl-Coa Thiolase antibody
Anti-Scp-2/Thiolase antibody
Anti-Scp-Chi antibody
Anti-Scpx antibody
Anti-Sterol Carrier Protein X antibody
Anti-Scp-X antibody
Anti-Straight-Chain Acyl-Coa Oxidase antibody
Anti-Scox antibody
Anti-SCP2 antibody

Information sourced from Uniprot.org

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