Anti-SCNN1G antibody (ECD) (STJ13101760)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the extracellular domain of human SCNN1G conjugated to immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-SCNN1G (ECD) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | SCNN1G |
| Gene ID | 6340 |
| Uniprot ID | SCNNG_HUMAN |
| Immunogen | A synthetic peptide from the extracellular domain of human SCNN1G conjugated to immunogenic carrier protein was used as the antigen. |
| Immunogen Region | ECD |
| Specificity | Specific for SCNN1G. |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits. ENaC is activated through the proteolytic maturation of its subunits. Furin cleaves the SCNN1G subunit first, followed by cleavage by prostasin (PRSS8), which results in a stepwise increase in the open probability of the channel due to the release of an inhibitory tract. BPIFA1, which is recruited by the SCNN1B subunit, prevents the proteolytic activation of ENaC. N-glycosylated. N-linked glycans are processed to complex type during ENaC complex assembly and transport to the plasma membrane. |
| Function | This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance. |
| Protein Name | Epithelial Sodium Channel Subunit GammaEnac Subunit GammaEnacgEpithelial Na(+ Channel Subunit GammaGamma-EnacAmiloride-Sensitive Sodium Channel Subunit GammaGamma-NachNonvoltage-Gated Sodium Channel 1 Subunit GammaScnegSodium Channel Epithelial 1 Subunit Gamma |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Epithelial Sodium Channel Subunit Gamma antibodyAnti-Enac Subunit Gamma antibodyAnti-Enacg antibodyAnti-Epithelial Na(+ Channel Subunit Gamma antibodyAnti-Gamma-Enac antibodyAnti-Amiloride-Sensitive Sodium Channel Subunit Gamma antibodyAnti-Gamma-Nach antibodyAnti-Nonvoltage-Gated Sodium Channel 1 Subunit Gamma antibodyAnti-Scneg antibodyAnti-Sodium Channel Epithelial 1 Subunit Gamma antibodyAnti-SCNN1G antibody |
Information sourced from Uniprot.org