Anti-SCNN1B antibody (581-630 aa) (STJ92916)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Nonvoltage-gated Sodium Channel 1 at the amino acid range 581-630
General Information
| Short Description | Rabbit polyclonal anti-Amiloride-sensitive sodium channel subunit beta channel subunit beta (581-630 aa) for use in IHC, IF, WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SCNN1B |
| Gene ID | 6338 |
| Uniprot ID | SCNNB_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Nonvoltage-gated Sodium Channel 1 at the amino acid range 581-630 |
| Immunogen Region | 581-630 aa |
| Specificity | ENaC Beta Polyclonal Antibody detects endogenous levels of ENaC Beta protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits. However, some studies could not confirm the ubiquitination of this subunit of the ENaC. Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. N-glycosylated. N-glycosylation is required for interaction with BPIFA1. |
| Function | This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance. |
| Protein Name | Epithelial Sodium Channel Subunit BetaBeta-EnacEnac Subunit BetaEnacbEpithelial Na(+ Channel Subunit BetaAmiloride-Sensitive Sodium Channel Subunit BetaBeta-NachNonvoltage-Gated Sodium Channel 1 Subunit BetaScneb |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle Membrane |
| Alternative Antibody Names | Anti-Epithelial Sodium Channel Subunit Beta antibodyAnti-Beta-Enac antibodyAnti-Enac Subunit Beta antibodyAnti-Enacb antibodyAnti-Epithelial Na(+ Channel Subunit Beta antibodyAnti-Amiloride-Sensitive Sodium Channel Subunit Beta antibodyAnti-Beta-Nach antibodyAnti-Nonvoltage-Gated Sodium Channel 1 Subunit Beta antibodyAnti-Scneb antibodyAnti-SCNN1B antibody |
Information sourced from Uniprot.org