Anti-SCNN1B antibody (570-640 aa) (STJ13101781)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 570-640 of human SCNN1B conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-SCNN1B (570-640 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (using recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | SCNN1B |
| Gene ID | 6338 |
| Uniprot ID | SCNNB_HUMAN |
| Immunogen | A synthetic peptide from aa region 570-640 of human SCNN1B conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 570-640 aa |
| Specificity | Specific for SCNN1B. |
Additional Info
| Post Translational Modifications | Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits. However, some studies could not confirm the ubiquitination of this subunit of the ENaC. Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. N-glycosylated. N-glycosylation is required for interaction with BPIFA1. |
| Function | This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance. |
| Protein Name | Epithelial Sodium Channel Subunit BetaBeta-EnacEnac Subunit BetaEnacbEpithelial Na(+ Channel Subunit BetaAmiloride-Sensitive Sodium Channel Subunit BetaBeta-NachNonvoltage-Gated Sodium Channel 1 Subunit BetaScneb |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle Membrane |
| Alternative Antibody Names | Anti-Epithelial Sodium Channel Subunit Beta antibodyAnti-Beta-Enac antibodyAnti-Enac Subunit Beta antibodyAnti-Enacb antibodyAnti-Epithelial Na(+ Channel Subunit Beta antibodyAnti-Amiloride-Sensitive Sodium Channel Subunit Beta antibodyAnti-Beta-Nach antibodyAnti-Nonvoltage-Gated Sodium Channel 1 Subunit Beta antibodyAnti-Scneb antibodyAnti-SCNN1B antibody |
Information sourced from Uniprot.org