Anti-SCNN1A antibody (320-400 aa) (STJ190541)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 320-400
General Information
| Short Description | Rabbit polyclonal anti-Amiloride-sensitive sodium channel subunit alpha channel subunit alpha (320-400 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SCNN1A |
| Gene ID | 6337 |
| Uniprot ID | SCNNA_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 320-400 |
| Immunogen Region | 320-400 aa |
| Specificity | SCNNA Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits. ENaC is activated through the proteolytic maturation of its subunits. Furin cleaves the SCNN1A subunit, which results in a stepwise increase in the open probability of the channel due to the release of an inhibitory tract. BPIFA1, which is recruited by the SCNN1B subunit, prevents the proteolytic activation of ENaC. N-glycosylated. |
| Function | This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance. Isoform 4: Not functional. |
| Protein Name | Epithelial Sodium Channel Subunit AlphaAlpha-EnacEnac Subunit AlphaEnacaEpithelial Na(+ Channel Subunit AlphaAlpha-NachAmiloride-Sensitive Sodium Channel Subunit AlphaNonvoltage-Gated Sodium Channel 1 Subunit AlphaScneaSodium Channel Epithelial 1 Subunit Alpha |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumCytoplasmic GranuleCytoplasmCytoplasmic VesicleSecretory VesicleAcrosomeFlagellumIn The Oviduct And BronchusLocated On Cilia In Multi-Ciliated CellsIn Endometrial Non-Ciliated Epithelial CellsRestricted To Apical SurfacesIn EpidermisLocated Nearly Uniformly In The Cytoplasm In A Granular DistributionIn Sebaceous GlandsObserved Only In The Cytoplasmic Space In Between The Lipid VesiclesIn Eccrine Sweat GlandsMainly Located At The Apical Surface Of The Cells Facing The LumenIn SkinIn Arrector Pili Muscle Cells And In AdipocytesLocated In The Cytoplasm And Colocalized With Actin FibersIn SpermatogoniaSpermatocytes And Round SpermatidsLocated In The CytoplasmPrior To SpermiationLocation Shifts From The Cytoplasm To The Spermatid TailIn SpermatozoaLocalizes At The Acrosome And The Central Region Of The Sperm Flagellum |
| Alternative Antibody Names | Anti-Epithelial Sodium Channel Subunit Alpha antibodyAnti-Alpha-Enac antibodyAnti-Enac Subunit Alpha antibodyAnti-Enaca antibodyAnti-Epithelial Na(+ Channel Subunit Alpha antibodyAnti-Alpha-Nach antibodyAnti-Amiloride-Sensitive Sodium Channel Subunit Alpha antibodyAnti-Nonvoltage-Gated Sodium Channel 1 Subunit Alpha antibodyAnti-Scnea antibodyAnti-Sodium Channel Epithelial 1 Subunit Alpha antibodyAnti-SCNN1A antibodyAnti-SCNN1 antibody |
Information sourced from Uniprot.org