Anti-SCN5A antibody (Internal) (STJ70769)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SCN5A (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000.IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5-8µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SCN5A |
| Gene ID | 6331 |
| Uniprot ID | SCN5A_HUMAN |
| Accession Number | NP_932173.1; NP_000326.2; NP_001092874.1; NP_001092875.1; NP_001153632.1; NP_001153633.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | ETDDQSPEKIN |
| Specificity | This antibody is expected to recognise all reported isoforms (NP_932173.1; NP_000326.2; NP_001092874.1; NP_001092875.1). |
Additional Info
| Post Translational Modifications | Ubiquitinated by NEDD4L.which promotes its endocytosis. Does not seem to be ubiquitinated by NEDD4 or WWP2. Phosphorylation at Ser-1503 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents (Probable). Regulated through phosphorylation by CaMK2D. Lacks the cysteine which covalently binds the conotoxin GVIIJ. This cysteine (position 868) is speculated in other sodium channel subunits alpha to be implied in covalent binding with the sodium channel subunit beta-2 or beta-4. N-glycosylated at Asn-318, probably hinders potential interaction with regulatory subunits. |
| Function | Pore-forming subunit of Nav1.5, a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na(+) ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues. Nav1.5 is the predominant sodium channel expressed in myocardial cells and it is responsible for the initial upstroke of the action potential in cardiac myocytes, thereby initiating the heartbeat. Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2. |
| Protein Name | Sodium Channel Protein Type 5 Subunit AlphaSodium Channel Protein Cardiac Muscle Subunit AlphaSodium Channel Protein Type V Subunit AlphaVoltage-Gated Sodium Channel Subunit Alpha Nav1.5Hh1 |
| Database Links | Reactome: R-HSA-445095Reactome: R-HSA-5576892 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmPerinuclear RegionSarcolemmaT-TubuleCell JunctionRangrf Promotes Trafficking To The Cell MembraneColocalizes With Pkp2 At Intercalated Disks In The Heart |
| Alternative Antibody Names | Anti-Sodium Channel Protein Type 5 Subunit Alpha antibodyAnti-Sodium Channel Protein Cardiac Muscle Subunit Alpha antibodyAnti-Sodium Channel Protein Type V Subunit Alpha antibodyAnti-Voltage-Gated Sodium Channel Subunit Alpha Nav1.5 antibodyAnti-Hh1 antibodyAnti-SCN5A antibody |
Information sourced from Uniprot.org