• Immunofluorescence analysis of L929 cells using SCIN Rabbit polyclonal antibody (STJ119829) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SCIN Rabbit polyclonal antibody (STJ119829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SCIN antibody (1-100) (STJ119829)

SKU:
STJ119829

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCIN (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCIN
Gene ID: 85477
Uniprot ID: SCIN_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SCIN (NP_001106177.1).
Immunogen Sequence: MARELYHEEFARAGKQAGLQ VWRIEKLELVPVPQSAHGDF YVGDAYLVLHTAKTSRGFTY HLHFWLGKECSQDESTAAAI FTVQMDDYLGGKPVQNRELQ
Tissue Specificity Expressed in megakaryocytes.
Function Ca(2+)-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2). In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles. Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts. Regulates chondrocyte proliferation and differentiation. Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways.
Protein Name Scinderin
Adseverin
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Podosome
Alternative Antibody Names Anti-Scinderin antibody
Anti-Adseverin antibody
Anti-SCIN antibody
Anti-KIAA1905 antibody

Information sourced from Uniprot.org

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