Anti-SCIMP antibody [NVL-07] (STJ16103394)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenRecombinant intracellular part of human SCIMP
General Information
| Short Description | Mouse monoclonal anti-SCIMP for use in FC, IP, WB and ICC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IP/WB/ICC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | NVL-07 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by protein-A affinity chromatography. |
| Formulation | Phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide |
| Storage Instruction | Store at 2-8°C for up to 1-year, upon receipt. |
Target Information
| Gene Symbol | SCIMP |
| Gene ID | 388325 |
| Uniprot ID | SCIMP_HUMAN |
| Immunogen | Recombinant intracellular part of human SCIMP |
Additional Info
| Post Translational Modifications | Phosphorylated by the Src-family protein tyrosine kinases LYN and SRC. Phosphorylation occurs on tyrosine residues upon MHC-II stimulation. Phosphorylation also occurs on tyrosine residues after activation of CLEC7A/DECTIN1 by particulate beta-glucan. Lipopolysaccharide (LPS) induces phosphorylation of Tyr-69, Tyr-107 and Tyr-131 differentially to allow temporal recruitment of effector proteins GRB2, CSK and BLNK. Phosphorylation of Tyr-69 is immediately induced by LPS stimulation and allows GRB2 to bind. Tyr-107 is phosphorylated 5 minutes after LPS stimulation, which then allows CSK to bind, followed by phosphorylation of Tyr-131 10 minutes after LPS induction, which allows BLNK to bind. Phosphorylation at Tyr-107 by LYN occurs after activation of TLR4 by lipopolysaccharide.phosphorylation enhances binding to TLR4. Palmitoylated. |
| Function | Lipid tetraspanin-associated transmembrane adapter/mediator that acts as a scaffold for Src-family kinases and other signaling proteins in immune cells. It is involved in major histocompatibility complex class II (MHC-II) signaling transduction in B cells, where it is required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation. In dendritic cells, it is involved in sustaining CLEC7A/DECTIN1 signaling after CLEC7A activation by fungal beta-glucans. It also acts as an agonist-inducible signaling adapter for TLR1, TLR2, TLR3, TLR4, and TLR7 by selectively enabling the expression of pro-inflammatory cytokines IL6 and IL12B in macrophages and acting as a scaffold for phosphorylation of Toll-like receptors by Src-family kinases. |
| Protein Name | Slp Adapter And Csk-Interacting Membrane ProteinSlp65/Slp76 - Csk-Interacting Membrane Protein |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinLipid-AnchorCytoplasmic VesiclePhagosomeCell ProjectionRuffleFilopodiumTogether With Mhc-IiAssociates With Lipid-Enriched Microdomains Called Tetraspanin-Enriched Microdomains (Tems)Rapidly Translocates Into Immunological Synapse (Is) At Cell-Cell Contacts Between Antigen-Presenting Cells (Apcs) And T-CellsColocalized With Tetraspanins Cd37Cd53And Cd81 At The UropodPresent At Regions Of Cell-Cell Contacts But Also At The Leading Edge Of Migrating CellsLocalizes To Phagosomes In Dendritic Cells After Exposure To Particulate Beta-Glucan |
| Alternative Antibody Names | Anti-Slp Adapter And Csk-Interacting Membrane Protein antibodyAnti-Slp65/Slp76 - Csk-Interacting Membrane Protein antibodyAnti-SCIMP antibodyAnti-C17orf87 antibodyAnti-UNQ5783 antibodyAnti-PRO16090 antibody |
Information sourced from Uniprot.org