• Western blot analysis of extracts of various cell lines, using SCG2 antibody (STJ28508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of human pancreas cells using SCG2 rabbit polyclonal antibody (STJ28508) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat pancreas cells using SCG2 rabbit polyclonal antibody (STJ28508) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SCG2 antibody (30-320) (STJ28508)

SKU:
STJ28508

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCG2 (30-320) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCG2
Gene ID: 7857
Uniprot ID: SCG2_HUMAN
Immunogen Region: 30-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-320 of human SCG2 (NP_003460.2).
Immunogen Sequence: FQRNQLLQKEPDLRLENVQK FPSPEMIRALEYIENLRQQA HKEESSPDYNPYQGVSVPLQ QKENGDESHLPERDSLSEED WMRIILEALRQAENEPQSAP KENKPYALNSEKNFPMDMSD DYETQQWPERKLKHMQFPPM YEENSRDNPFKRTNEIVEEQ YTPQSLATLESVFQELGKLT GPNNQKRERMDEEQKLYTDD EDDIYKANNIAYEDVVGGED WNPVEEKIESQTQEEVRDS
Post Translational Modifications O-glycosylated.
Function Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules.
Protein Name Secretogranin-2
Chromogranin-C
Secretogranin Ii
Sgii Cleaved Into - Secretoneurin
Sn - Manserin
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Neuroendocrine And Endocrine Secretory Granules
Alternative Antibody Names Anti-Secretogranin-2 antibody
Anti-Chromogranin-C antibody
Anti-Secretogranin Ii antibody
Anti-Sgii Cleaved Into - Secretoneurin antibody
Anti-Sn - Manserin antibody
Anti-SCG2 antibody
Anti-CHGC antibody

Information sourced from Uniprot.org

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