• Western blot analysis of extracts of various cell lines, using SCD antibody (STJ118857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of U-251MG cells, using SCD antibody (STJ118857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SCD rabbit polyclonal antibody (STJ118857) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using SCD rabbit polyclonal antibody (STJ118857) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A431 cells using SCD rabbit polyclonal antibody (STJ118857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Anti-SCD antibody (25-43) (STJ118857)

Anti-SCD antibody (25-43) (STJ118857)

SKU:
STJ118857

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCD (25-43) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCD
Gene ID: 6319
Uniprot ID: SCD_HUMAN
Immunogen Region: 25-43
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 25-43 of human SCD (NP_005054.3).
Immunogen Sequence: SRVLQNGGDKLETMPLYLE
Tissue Specificity Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.
Function Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation. Plays an important role in body energy homeostasis. Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides.
Protein Name Stearoyl-Coa Desaturase
Hscd1
Acyl-Coa Desaturase
Delta(9-Desaturase
Delta-9 Desaturase
Fatty Acid Desaturase
Database Links Reactome: R-HSA-2426168
Reactome: R-HSA-75105
Reactome: R-HSA-9029558
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Stearoyl-Coa Desaturase antibody
Anti-Hscd1 antibody
Anti-Acyl-Coa Desaturase antibody
Anti-Delta(9-Desaturase antibody
Anti-Delta-9 Desaturase antibody
Anti-Fatty Acid Desaturase antibody
Anti-SCD antibody
Anti-FADS5 antibody
Anti-SCD1 antibody
Anti-SCDOS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance