• Western blot analysis of extracts of various cell lines, using SR-B2/LIMPII antibody (STJ114596) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L929 cells using SR-B2/LIMPII antibody (STJ114596) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U20S cells using SR-B2/LIMPII antibody (STJ114596) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SCARB2 antibody (200-380) (STJ114596)

SKU:
STJ114596

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCARB2 (200-380) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCARB2
Gene ID: 950
Uniprot ID: SCRB2_HUMAN
Immunogen Region: 200-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human SR-B2/LIMPII (NP_005497.1).
Immunogen Sequence: GLFYEKNGTNDGDYVFLTGE DSYLNFTKIVEWNGKTSLDW WITDKCNMINGTDGDSFHPL ITKDEVLYVFPSDFCRSVYI TFSDYESVQGLPAFRYKVPA EILANTSDNAGFCIPEGNCL GSGVLNVSICKNGAPIIMSF PHFYQADERFVSAIEGMHPN QEDHETFVDINPLTGIILKA A
Function Acts as a lysosomal receptor for glucosylceramidase (GBA1) targeting. (Microbial infection) Acts as a receptor for enterovirus 71.
Protein Name Lysosome Membrane Protein 2
85 Kda Lysosomal Membrane Sialoglycoprotein
Lgp85
Cd36 Antigen-Like 2
Lysosome Membrane Protein Ii
Limp Ii
Scavenger Receptor Class B Member 2
Cd Antigen Cd36
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Lysosome Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Lysosome Membrane Protein 2 antibody
Anti-85 Kda Lysosomal Membrane Sialoglycoprotein antibody
Anti-Lgp85 antibody
Anti-Cd36 Antigen-Like 2 antibody
Anti-Lysosome Membrane Protein Ii antibody
Anti-Limp Ii antibody
Anti-Scavenger Receptor Class B Member 2 antibody
Anti-Cd Antigen Cd36 antibody
Anti-SCARB2 antibody
Anti-CD36L2 antibody
Anti-LIMP2 antibody
Anti-LIMPII antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance