| Host: | Goat |
| Applications: | Pep-ELISA/WB/IP/IHC/ICC/IF |
| Reactivity: | Human/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SBP2 (Internal) is suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SECISBP2 |
| Gene ID: | 79048 |
| Uniprot ID: | SEBP2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_076982.3 |
| Immunogen Sequence: | KERQERKQRLQEN |
| Function | mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid selenocysteine. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. |
| Protein Name | Selenocysteine Insertion Sequence-Binding Protein 2Secis-Binding Protein 2 |
| Database Links | Reactome: R-HSA-2408557 |
| Cellular Localisation | Isoform 1: NucleusIsoform 2: Mitochondrion |
| Alternative Antibody Names | Anti-Selenocysteine Insertion Sequence-Binding Protein 2 antibodyAnti-Secis-Binding Protein 2 antibodyAnti-SECISBP2 antibodyAnti-SBP2 antibody |
Information sourced from Uniprot.org