• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike RBD antibody (STJ11101792). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike RBD antibody (STJ11101792) at a dilution of 1:3000.
  • Immunofluorescence analysis of 293T-RBD and 293T cells using SARS-CoV-2 Spike RBD Rabbit polyclonal antibody (STJ11101792) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immobilized Recombinant SARS-COV-2 Spike RBD Protein (RP01278LQ) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike RBD Rabbit polyclonal antibody (STJ11101792) with a linear range of 0. 78-50ng/mL.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike RBD Protein, using SARS-CoV-2 Spike RBD Rabbit polyclonal antibody (STJ11101792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SARS-CoV-2 Spike RBD antibody (332-550) (STJ11101792)

SKU:
STJ11101792

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:1:50000-1:200000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 332-550
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 332-550 of coronavirus Spike RBD (YP_009724390.1).
Immunogen Sequence: ITNLCPFGEVFNATRFASVY AWNRKRISNCVADYSVLYNS ASFSTFKCYGVSPTKLNDLC FTNVYADSFVIRGDEVRQIA PGQTGKIADYNYKLPDDFTG CVIAWNSNNLDSKVGGNYNY LYRLFRKSNLKPFERDISTE IYQAGSTPCNGVEGFNCYFP LQSYGFQPTNGVGYQPYRVV VLSFELLHAPATVCGPKKST NLVKNKCVNFNFNGLTGTG
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E) , spike or surface glycoprotein (S) , membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Information sourced from Uniprot.org

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