• Immunohistochemistry analysis of paraffin-embedded human appendix using SAMHD1 Rabbit monoclonal antibody (STJ11103570) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (STJ11103570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SAMHD1 antibody (527-626) [S0MR] (STJ11103570)

SKU:
STJ11103570

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SAMHD1 (527-626) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAMHD1
Gene ID: 25939
Uniprot ID: SAMH1_HUMAN
Immunogen Region: 527-626
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 527-626 of human SAMHD1 (NP_056289.2).
Immunogen Sequence: NRAIRITKNQVSQLLPEKFA EQLIRVYCKKVDRKSLYAAR QYFVQWCADRNFTKPQDGDV IAPLITPQKKEWNDSTSVQN PTRLREASKSRVQLFKDDPM
Tissue Specificity Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
Post Translational Modifications Phosphorylation at Thr-592 by CDK1 acts as a switch to control deoxynucleoside triphosphate (dNTPase)-dependent and -independent functions. Phosphorylation at Thr-592 takes place in cycling cells: it reduces the stability of the homotetramer, impairing the dNTPase activity and subsequent ability to restrict infection by viruses. It also inhibits ability to suppress LINE-1 retrotransposon activity. In contrast, phosphorylation at Thr-592 promotes DNA end resection at stalled replication forks in response to DNA damage. (Microbial infection) Phosphorylation at Thr-592 by Epstein-Barr virus kinase BGLF4 and human cytomegalovirus/HCMV UL97 leads to a reduced level of dCTPase and dTTPase activity and the loss of viral restriction. (Microbial infection) Ubiquitinated following interaction with HIV-2 viral protein Vpx.Vpx promotes interaction and with a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase, leading to proteasomal degradation.
Function Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. Not able to restrict infection by HIV-2 virus.because restriction activity is counteracted by HIV-2 viral protein Vpx. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools. Phosphorylation at Thr-592 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks. Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication. Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation. Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity. Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation.
Protein Name Deoxynucleoside Triphosphate Triphosphohydrolase Samhd1
Dntpase
Dendritic Cell-Derived Ifng-Induced Protein
Dcip
Monocyte Protein 5
Mop-5
Sam Domain And Hd Domain-Containing Protein 1
Hsamhd1
Database Links Reactome: R-HSA-8956319
Reactome: R-HSA-909733
Cellular Localisation Nucleus
Chromosome
Localizes To Sites Of Dna Double-Strand Breaks In Response To Dna Damage
Alternative Antibody Names Anti-Deoxynucleoside Triphosphate Triphosphohydrolase Samhd1 antibody
Anti-Dntpase antibody
Anti-Dendritic Cell-Derived Ifng-Induced Protein antibody
Anti-Dcip antibody
Anti-Monocyte Protein 5 antibody
Anti-Mop-5 antibody
Anti-Sam Domain And Hd Domain-Containing Protein 1 antibody
Anti-Hsamhd1 antibody
Anti-SAMHD1 antibody
Anti-MOP5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance