Anti-SAE1/AOS1 antibody (C-Term) (STJ70426)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-SAE1/AOS1 (C-Term) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/FC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 37kDa band observed in lysates of cell lines HEK293 and A549, and approx. 38-40kDa in lysates of cell line Jurkat (calculated MW of 38.4kDa according to NP_005491.1). Recommend |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | SAE1 |
| Gene ID | 10055 |
| Uniprot ID | SAE1_HUMAN |
| Accession Number | NP_005491.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | MKGNGIVECLGPK |
Additional Info
| Function | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Protein Name | Sumo-Activating Enzyme Subunit 1Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-3065676Reactome: R-HSA-3065678 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Sumo-Activating Enzyme Subunit 1 antibodyAnti-Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed antibodyAnti-SAE1 antibodyAnti-AOS1 antibodyAnti-SUA1 antibodyAnti-UBLE1A antibody |
Information sourced from Uniprot.org