• STJ72798 (0. 03µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-S100A8 antibody (Internal) (STJ72798)

SKU:
STJ72798-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-S100A8 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.03-0.1µg/ml
IHC-5µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: S100A8
Gene ID: 6279
Uniprot ID: S10A8_HUMAN
Immunogen Region: Internal
Accession Number: NP_002955.2
Immunogen Sequence: KGADVWFKELDINT
Function S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect.regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a IL-x-C-x-x-DE motif.S100A8 seems to contribute to S-nitrosylation site selectivity. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner.
Protein Name Protein S100-A8
Calgranulin-A
Calprotectin L1l Subunit
Cystic Fibrosis Antigen
Cfag
Leukocyte L1 Complex Light Chain
Migration Inhibitory Factor-Related Protein 8
Mrp-8
P8
S100 Calcium-Binding Protein A8
Urinary Stone Protein Band A
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-166058
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5668599
Reactome: R-HSA-5686938
Reactome: R-HSA-6798695
Reactome: R-HSA-6799990
Cellular Localisation Secreted
Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Predominantly Localized In The Cytoplasm
Upon Elevation Of The Intracellular Calcium Level
Translocated From The Cytoplasm To The Cytoskeleton And The Cell Membrane
Upon Neutrophil Activation Or Endothelial Adhesion Of Monocytes
Is Secreted Via A Microtubule-Mediated
Alternative Pathway
Alternative Antibody Names Anti-Protein S100-A8 antibody
Anti-Calgranulin-A antibody
Anti-Calprotectin L1l Subunit antibody
Anti-Cystic Fibrosis Antigen antibody
Anti-Cfag antibody
Anti-Leukocyte L1 Complex Light Chain antibody
Anti-Migration Inhibitory Factor-Related Protein 8 antibody
Anti-Mrp-8 antibody
Anti-P8 antibody
Anti-S100 Calcium-Binding Protein A8 antibody
Anti-Urinary Stone Protein Band A antibody
Anti-S100A8 antibody
Anti-CAGA antibody
Anti-CFAG antibody
Anti-MRP8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance