• Confocal imaging of C2C12 cells using S100A6 Rabbit monoclonal antibody (STJ11101874, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of S100A6 in paraffin-embedded human liver using S100A6 Rabbit monoclonal antibody (STJ11101874) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of S100A6 in paraffin-embedded rat spleen using S100A6 Rabbit monoclonal antibody (STJ11101874) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using S100A6 Rabbit monoclonal antibody (STJ11101874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using S100A6 Rabbit monoclonal antibody (STJ11101874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-S100A6 antibody (1-90) [S4MR] (STJ11101874)

SKU:
STJ11101874

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-S100 alpha 6 (1-90) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A6
Gene ID: 6277
Uniprot ID: S10A6_HUMAN
Immunogen Region: 1-90
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (P06703).
Immunogen Sequence: MACPLDQAIGLLVAIFHKYS GREGDKHTLSKKELKELIQK ELTIGSKLQDAEIARLMEDL DRNKDQEVNFQEYVTFLGAL ALIYNEALKG
Post Translational Modifications The N-terminus is blocked.
Function May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
Protein Name Protein S100-A6
Calcyclin
Growth Factor-Inducible Protein 2a9
Mln 4
Prolactin Receptor-Associated Protein
Pra
S100 Calcium-Binding Protein A6
Cellular Localisation Nucleus Envelope
Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Protein S100-A6 antibody
Anti-Calcyclin antibody
Anti-Growth Factor-Inducible Protein 2a9 antibody
Anti-Mln 4 antibody
Anti-Prolactin Receptor-Associated Protein antibody
Anti-Pra antibody
Anti-S100 Calcium-Binding Protein A6 antibody
Anti-S100A6 antibody
Anti-CACY antibody

Information sourced from Uniprot.org

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