![Western blot analysis of various lysates using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100473/386998/STJ25432_1__96427.1713156179.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of lysates from wild type (WT) and S100A6 knockout (KO) HeLa cells, using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100473/386999/STJ25432_2__75971.1713156180.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and S100A6 knockout (KO) HeLa cells, using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western blot analysis of various lysates using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/100473/386998/STJ25432_1__96427.1713156179.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
| Short Description : | Rabbit polyclonal S100A6 (1-90) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | S100A6 |
| Gene ID: | 6277 |
| Uniprot ID: | S10A6_HUMAN |
| Immunogen Region: | 1-90 |
| Immunogen Sequence: | MACPLDQAIGLLVAIFHKYS GREGDKHTLSKKELKELIQK ELTIGSKLQDAEIARLMEDL DRNKDQEVNFQEYVTFLGAL ALIYNEALKG |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1). |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. |
| Protein Name | Protein S100-A6CalcyclinGrowth Factor-Inducible Protein 2a9Mln 4Prolactin Receptor-Associated ProteinPraS100 Calcium-Binding Protein A6 |
| Database Links | |
| Cellular Localisation | Nucleus EnvelopeCytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Protein S100-A6 antibodyAnti-Calcyclin antibodyAnti-Growth Factor-Inducible Protein 2a9 antibodyAnti-Mln 4 antibodyAnti-Prolactin Receptor-Associated Protein antibodyAnti-Pra antibodyAnti-S100 Calcium-Binding Protein A6 antibodyAnti-S100A6 antibodyAnti-CACY antibody |
Information sourced from Uniprot.org
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