• Immunohistochemistry analysis of S100A5 in paraffin-embedded human colon using S100A5 Rabbit polyclonal antibody (STJ116979) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of S100A5 in paraffin-embedded human thyroid cancer using S100A5 Rabbit polyclonal antibody (STJ116979) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from U-251MG cells, using S100A5 Rabbit polyclonal antibody (STJ116979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-S100A5 antibody (1-92) (STJ116979)

SKU:
STJ116979

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-S100A5 (1-92) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A5
Gene ID: 6276
Uniprot ID: S10A5_HUMAN
Immunogen Region: 1-92
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A5 (NP_002953.2).
Immunogen Sequence: METPLEKALTTMVTTFHKYS GREGSKLTLSRKELKELIKK ELCLGEMKESSIDDLMKSLD KNSDQEIDFKEYSVFLTMLC MAYNDFFLEDNK
Function Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.
Protein Name Protein S100-A5
Protein S-100d
S100 Calcium-Binding Protein A5
Alternative Antibody Names Anti-Protein S100-A5 antibody
Anti-Protein S-100d antibody
Anti-S100 Calcium-Binding Protein A5 antibody
Anti-S100A5 antibody
Anti-S100D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance