Confocal imaging ofย paraffin-embedded Human colon usingย FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-S100A4 antibody (2-101) [S6MR] (STJ11101646)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101646
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit monoclonal FSP1/S100A4 (2-101) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS6MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:2000
IHC-P:1:200-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolS100A4
Gene ID6275
Uniprot IDS10A4_HUMAN
Immunogen Region2-101
Immunogen SequenceACPLEKALDVMVSTFHKYSG KEGDKFKLNKSELKELLTRE LPSFLGKRTDEAAFQKLMSN LDSNRDNEVDFQEYCVFLSC IAMMCNEFFEGFPDKQPRKK
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 2-101 of human S100A4 (P26447).

Additional Info

Tissue Specificity Ubiquitously expressed.
Function Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9. Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis. Also modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels. Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes. In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors.
Protein Name Protein S100-A4
Calvasculin
Metastasin
Placental Calcium-Binding Protein
Protein Mts1
S100 Calcium-Binding Protein A4
Database Links
Cellular Localisation Secreted
Nucleus
Cytoplasm
Alternative Antibody Names Anti-Protein S100-A4 antibody
Anti-Calvasculin antibody
Anti-Metastasin antibody
Anti-Placental Calcium-Binding Protein antibody
Anti-Protein Mts1 antibody
Anti-S100 Calcium-Binding Protein A4 antibody
Anti-S100A4 antibody
Anti-CAPL antibody
Anti-MTS1 antibody

Information sourced from Uniprot.org

Citations

Product Review

!