• Confocal imaging of paraffin-embedded Human colon using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of NIH-3T3 cells using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human colon tissue using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human liver tissue using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris-EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-S100A4 antibody (2-101) [S6MR] (STJ11101646)

SKU:
STJ11101646

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FSP1/S100A4 (2-101) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A4
Gene ID: 6275
Uniprot ID: S10A4_HUMAN
Immunogen Region: 2-101
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-101 of human S100A4 (P26447).
Immunogen Sequence: ACPLEKALDVMVSTFHKYSG KEGDKFKLNKSELKELLTRE LPSFLGKRTDEAAFQKLMSN LDSNRDNEVDFQEYCVFLSC IAMMCNEFFEGFPDKQPRKK
Tissue Specificity Ubiquitously expressed.
Function Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9. Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis. Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels. Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes. In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors.
Protein Name Protein S100-A4
Calvasculin
Metastasin
Placental Calcium-Binding Protein
Protein Mts1
S100 Calcium-Binding Protein A4
Cellular Localisation Secreted
Nucleus
Cytoplasm
Alternative Antibody Names Anti-Protein S100-A4 antibody
Anti-Calvasculin antibody
Anti-Metastasin antibody
Anti-Placental Calcium-Binding Protein antibody
Anti-Protein Mts1 antibody
Anti-S100 Calcium-Binding Protein A4 antibody
Anti-S100A4 antibody
Anti-CAPL antibody
Anti-MTS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance