• Western blot analysis of extracts of various cell lines, using S100/S100A1 antibody (STJ11101644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-S100A1 antibody (2-94) [S4MR] (STJ11101644)

SKU:
STJ11101644

$197.78 - $863.33
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-S100 (2-94) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A1
Gene ID: 6271
Uniprot ID: S10A1_HUMAN
Immunogen Region: 2-94
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-94 of human S100/S100A1 (P23297).
Immunogen Sequence: GSELETAMETLINVFHAHSG KEGDKYKLSKKELKELLQTE LSGFLDAQKDVDAVDKVMKE LDENGDGEVDFQEYVVLVAA LTVACNNFFWENS
Tissue Specificity Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Post Translational Modifications Glutathionylated.glutathionylation increases affinity to calcium about 10-fold.
Function Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole.
Protein Name Protein S100-A1
S-100 Protein Alpha Chain
S-100 Protein Subunit Alpha
S100 Calcium-Binding Protein A1
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-166058
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Cellular Localisation Cytoplasm
Sarcoplasmic Reticulum
Mitochondrion
Alternative Antibody Names Anti-Protein S100-A1 antibody
Anti-S-100 Protein Alpha Chain antibody
Anti-S-100 Protein Subunit Alpha antibody
Anti-S100 Calcium-Binding Protein A1 antibody
Anti-S100A1 antibody
Anti-S100A antibody

Information sourced from Uniprot.org

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