• Western blot analysis of extracts of various cell lines, using RUVBL1/TIP49A/PONTIN rabbit monoclonal antibody (STJ11102285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using RUVBL1/TIP49A/PONTIN rabbit monoclonal antibody (STJ11102285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN rabbit monoclonal antibody antibody (STJ11102285) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RUVBL1 antibody (350-456) [S5MR] (STJ11102285)

SKU:
STJ11102285

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TIP49A (350-456) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RUVBL1
Gene ID: 8607
Uniprot ID: RUVB1_HUMAN
Immunogen Region: 350-456
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-456 of human RUVBL1/TIP49A/PONTIN (Q9Y265).
Immunogen Sequence: IPLDLLDRVMIIRTMLYTPQ EMKQIIKIRAQTEGINISEE ALNHLGEIGTKTTLRYSVQL LTPANLLAKINGKDSIEKEH VEEISELFYDAKSSAKILAD QQDKYMK
Tissue Specificity Ubiquitously expressed with high expression in heart, skeletal muscle and testis.
Function Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity.hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation. May be able to bind plasminogen at cell surface and enhance plasminogen activation.
Protein Name Ruvb-Like 1
49 Kda Tata Box-Binding Protein-Interacting Protein
49 Kda Tbp-Interacting Protein
54 Kda Erythrocyte Cytosolic Protein
Ecp-54
Ino80 Complex Subunit H
Nuclear Matrix Protein 238
Nmp 238
Pontin 52
Tip49a
Tip60-Associated Protein 54-Alpha
Tap54-Alpha
Database Links Reactome: R-HSA-171319
Reactome: R-HSA-201722
Reactome: R-HSA-3214847
Reactome: R-HSA-5689603
Reactome: R-HSA-5689880
Reactome: R-HSA-5696394
Reactome: R-HSA-606279
Cellular Localisation Nucleus Matrix
Nucleus
Nucleoplasm
Cytoplasm
Membrane
Cytoskeleton
Microtubule Organizing Center
Centrosome
Dynein Axonemal Particle
Mainly Localized In The Nucleus
Associated With Nuclear Matrix Or In The Nuclear Cytosol
Although It Is Also Present In The Cytoplasm And Associated With The Cell Membranes
In Prophase And Prometaphase It Is Located At The Centrosome And The Branching Microtubule Spindles
After Mitotic Nuclear Membrane Disintigration It Accumulates At The Centrosome And Sites Of Tubulin Polymerization
As Cells Pass Through Metaphase And Into Telophase It Is Located Close To The Centrosome At The Early Phase Of Tubulin Polymerization
In Anaphase It Accumulates At The Zone Of Tubule Interdigitation
In Telophase It Is Found At Polar Tubule Overlap
And It Reappears At The Site Of Chromosomal Decondensation In The Daughter Cells
Alternative Antibody Names Anti-Ruvb-Like 1 antibody
Anti-49 Kda Tata Box-Binding Protein-Interacting Protein antibody
Anti-49 Kda Tbp-Interacting Protein antibody
Anti-54 Kda Erythrocyte Cytosolic Protein antibody
Anti-Ecp-54 antibody
Anti-Ino80 Complex Subunit H antibody
Anti-Nuclear Matrix Protein 238 antibody
Anti-Nmp 238 antibody
Anti-Pontin 52 antibody
Anti-Tip49a antibody
Anti-Tip60-Associated Protein 54-Alpha antibody
Anti-Tap54-Alpha antibody
Anti-RUVBL1 antibody
Anti-INO80H antibody
Anti-NMP238 antibody
Anti-TIP49 antibody
Anti-TIP49A antibody

Information sourced from Uniprot.org

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