Anti-RUNX2 antibody [R08-7F3] (STJA0036143)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human RUNX2
General Information
| Short Description | Rabbit monoclonal anti-RUNX2 for use in IHC-P, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-7F3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RUNX2 |
| Gene ID | 860 |
| Uniprot ID | RUNX2_HUMAN |
| Immunogen | A synthesized peptide derived from human RUNX2 |
Additional Info
| Tissue Specificity | Specifically expressed in osteoblasts. |
| Post Translational Modifications | Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation. Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340. |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. |
| Protein Name | Runt-Related Transcription Factor 2Acute Myeloid Leukemia 3 ProteinCore-Binding Factor Subunit Alpha-1Cbf-Alpha-1Oncogene Aml-3Osteoblast-Specific Transcription Factor 2Osf-2Polyomavirus Enhancer-Binding Protein 2 Alpha A SubunitPea2-Alpha APebp2-Alpha ASl3-3 Enhancer Factor 1 Alpha A SubunitSl3/Akv Core-Binding Factor Alpha A Subunit |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-8878166Reactome: R-HSA-8939246Reactome: R-HSA-8939902Reactome: R-HSA-8940973Reactome: R-HSA-8941284Reactome: R-HSA-8941326Reactome: R-HSA-8941332Reactome: R-HSA-8941333 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Runt-Related Transcription Factor 2 antibodyAnti-Acute Myeloid Leukemia 3 Protein antibodyAnti-Core-Binding Factor Subunit Alpha-1 antibodyAnti-Cbf-Alpha-1 antibodyAnti-Oncogene Aml-3 antibodyAnti-Osteoblast-Specific Transcription Factor 2 antibodyAnti-Osf-2 antibodyAnti-Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit antibodyAnti-Pea2-Alpha A antibodyAnti-Pebp2-Alpha A antibodyAnti-Sl3-3 Enhancer Factor 1 Alpha A Subunit antibodyAnti-Sl3/Akv Core-Binding Factor Alpha A Subunit antibodyAnti-RUNX2 antibodyAnti-AML3 antibodyAnti-CBFA1 antibodyAnti-OSF2 antibodyAnti-PEBP2A antibody |
Information sourced from Uniprot.org