Anti-Runx2 antibody (Internal Region) (STJ13101931)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from internal region of mouse Runx2 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in human and rat.
General Information
| Short Description | Rabbit polyclonal anti-Runx2 (Internal Region) for use in WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | WB. A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | RUNX2 |
| Gene ID | 860 |
| Uniprot ID | RUNX2_HUMAN |
| Immunogen | A synthetic peptide from internal region of mouse Runx2 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in human and rat. |
| Immunogen Region | Internal Region |
| Specificity | Specific for Runx2. |
Additional Info
| Post Translational Modifications | Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation. Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340. |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. |
| Protein Name | Runt-Related Transcription Factor 2Acute Myeloid Leukemia 3 ProteinCore-Binding Factor Subunit Alpha-1Cbf-Alpha-1Oncogene Aml-3Osteoblast-Specific Transcription Factor 2Osf-2Polyomavirus Enhancer-Binding Protein 2 Alpha A SubunitPea2-Alpha APebp2-Alpha ASl3-3 Enhancer Factor 1 Alpha A SubunitSl3/Akv Core-Binding Factor Alpha A Subunit |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-8878166Reactome: R-HSA-8939246Reactome: R-HSA-8939902Reactome: R-HSA-8940973Reactome: R-HSA-8941284Reactome: R-HSA-8941326Reactome: R-HSA-8941332Reactome: R-HSA-8941333 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Runt-Related Transcription Factor 2 antibodyAnti-Acute Myeloid Leukemia 3 Protein antibodyAnti-Core-Binding Factor Subunit Alpha-1 antibodyAnti-Cbf-Alpha-1 antibodyAnti-Oncogene Aml-3 antibodyAnti-Osteoblast-Specific Transcription Factor 2 antibodyAnti-Osf-2 antibodyAnti-Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit antibodyAnti-Pea2-Alpha A antibodyAnti-Pebp2-Alpha A antibodyAnti-Sl3-3 Enhancer Factor 1 Alpha A Subunit antibodyAnti-Sl3/Akv Core-Binding Factor Alpha A Subunit antibodyAnti-RUNX2 antibodyAnti-AML3 antibodyAnti-CBFA1 antibodyAnti-OSF2 antibodyAnti-PEBP2A antibody |
Information sourced from Uniprot.org