• Western blot analysis of various lysates using RUNX1 Rabbit polyclonal antibody (STJ113542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-RUNX1 antibody (150-250) (STJ113542)

SKU:
STJ113542

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RUNX1 (150-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RUNX1
Gene ID: 861
Uniprot ID: RUNX1_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human RUNX1 (NP_001001890.1).
Immunogen Sequence: ITVFTNPPQVATYHRAIKIT VDGPREPRRHRQKLDDQTKP GSLSFSERLSELEQLRRTAM RVSPHHPAPTPNPRASLNHS TAFNPQPQSQMQDTRQIQPS P
Tissue Specificity Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
Post Translational Modifications Phosphorylated in its C-terminus upon IL-6 treatment. Phosphorylation enhances interaction with KAT6A. Methylated. Phosphorylated in Ser-249 Thr-273 and Ser-276 by HIPK2 when associated with CBFB and DNA. This phosphorylation promotes subsequent EP300 phosphorylation.
Function Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells. Positively regulates the expression of RORC in T-helper 17 cells. Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Isoform AML-1L interferes with the transactivation activity of RUNX1.
Protein Name Runt-Related Transcription Factor 1
Acute Myeloid Leukemia 1 Protein
Core-Binding Factor Subunit Alpha-2
Cbf-Alpha-2
Oncogene Aml-1
Polyomavirus Enhancer-Binding Protein 2 Alpha B Subunit
Pea2-Alpha B
Pebp2-Alpha B
Sl3-3 Enhancer Factor 1 Alpha B Subunit
Sl3/Akv Core-Binding Factor Alpha B Subunit
Database Links Reactome: R-HSA-1912408
Reactome: R-HSA-549127
Reactome: R-HSA-8877330
Reactome: R-HSA-8931987
Reactome: R-HSA-8934593
Reactome: R-HSA-8935964
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-8939242
Reactome: R-HSA-8939243
Reactome: R-HSA-8939245
Reactome: R-HSA-8939246
Reactome: R-HSA-8939247
Reactome: R-HSA-8939256
Reactome: R-HSA-8941333
Reactome: R-HSA-8951936
Reactome: R-HSA-9018519
Reactome: R-HSA-9616222
Reactome: R-HSA-9692916
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Runt-Related Transcription Factor 1 antibody
Anti-Acute Myeloid Leukemia 1 Protein antibody
Anti-Core-Binding Factor Subunit Alpha-2 antibody
Anti-Cbf-Alpha-2 antibody
Anti-Oncogene Aml-1 antibody
Anti-Polyomavirus Enhancer-Binding Protein 2 Alpha B Subunit antibody
Anti-Pea2-Alpha B antibody
Anti-Pebp2-Alpha B antibody
Anti-Sl3-3 Enhancer Factor 1 Alpha B Subunit antibody
Anti-Sl3/Akv Core-Binding Factor Alpha B Subunit antibody
Anti-RUNX1 antibody
Anti-AML1 antibody
Anti-CBFA2 antibody

Information sourced from Uniprot.org

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