Anti-RUFY1 antibody (Internal) (STJ72442)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-RUFY1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Recommended concentration: 0.05-0.2µg/ml. Primary incubation was 1 hour. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | RUFY1 |
| Gene ID | 80230 |
| Uniprot ID | RUFY1_HUMAN |
| Accession Number | NP_079434.3; NP_001035541.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | QKNEAITSFEGKT |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1. |
Additional Info
| Post Translational Modifications | Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting. |
| Function | Activating adapter involved in cargo sorting from early/recycling endosomes. Regulates retrieval of proteins from endosomes to the trans-Golgi network through interaction with the dynein-dynactin complex. Dual effector of RAB4B and RAB14, mediates a cooperative interaction allowing endosomal tethering and fusion. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. In oocytes, self-assembles to form a protein matrix which hold together endolysosomes, autophagosomes and proteasomes and generate non-membrane-bound compartments called endo-lysosomal vesicular assemblies (ELVAs). In immature oocytes, ELVAs sequester ubiquitinated protein aggregates and degrade them upon oocyte maturation. |
| Protein Name | Run And Fyve Domain-Containing Protein 1Fyve-Finger Protein Eip1La-Binding Protein 1Rab4-Interacting ProteinZinc Finger Fyve Domain-Containing Protein 12 |
| Database Links | Reactome: R-HSA-1660499 |
| Cellular Localisation | Early Endosome MembranePeripheral Membrane ProteinIn OocytesLocalizes In Non-Membrane-Bound Compartments Called Endo-Lysosomal Vesicular Assemblies (Elvas) |
| Alternative Antibody Names | Anti-Run And Fyve Domain-Containing Protein 1 antibodyAnti-Fyve-Finger Protein Eip1 antibodyAnti-La-Binding Protein 1 antibodyAnti-Rab4-Interacting Protein antibodyAnti-Zinc Finger Fyve Domain-Containing Protein 12 antibodyAnti-RUFY1 antibodyAnti-RABIP4 antibodyAnti-ZFYVE12 antibody |
Information sourced from Uniprot.org