• Western blot analysis of various lysates using Rubicon Rabbit polyclonal antibody (STJ119072) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RUBCN antibody (1-200) (STJ119072)

SKU:
STJ119072

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Rubicon (1-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RUBCN
Gene ID: 9711
Uniprot ID: RUBIC_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Rubicon (NP_055502.1).
Immunogen Sequence: MRPEGAGMELGGGEERLPEE SRREHWQLLGNLKTTVEGLV STNSPNVWSKYGGLERLCRD MQSILYHGLIRDQACRRQTD YWQFVKDIRWLSPHSALHVE KFISVHENDQSSADGASERA VAELWLQHSLQYHCLSAQLR PLLGDRQYIRKFYTDAAFLL SDAHVTAMLQCLEAVEQNNP RLLAQIDASMFARKHESPLL
Function Inhibits PIK3C3 activity.under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation. Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes.
Protein Name Run Domain Beclin-1-Interacting And Cysteine-Rich Domain-Containing Protein
Rubicon
Beclin-1 Associated Run Domain Containing Protein
Baron
Cellular Localisation Late Endosome
Lysosome
Early Endosome
Predominantly Located In Late Endosomes/Lysosomes
Only Partially Detected In Early Endosome And Not At All In The Golgi Apparatus
Alternative Antibody Names Anti-Run Domain Beclin-1-Interacting And Cysteine-Rich Domain-Containing Protein antibody
Anti-Rubicon antibody
Anti-Beclin-1 Associated Run Domain Containing Protein antibody
Anti-Baron antibody
Anti-RUBCN antibody
Anti-KIAA0226 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance