Anti-rRNA 2 O-methyltransferase fibrillarin antibody [38F3] (STJA0040745)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
ImmunogenYeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
General Information
| Short Description | Mouse monoclonal anti-rRNA 2 O-methyltransferase fibrillarin for use in ICC and WB in C.elegans, Drosophila, Human, Other Mammals, Rat and Yeast samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Mouse |
| Reactivity | C.elegans/Drosophila/Human/Other Mammals/Rat/Yeast |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 38F3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 1:500-1:10000 (see notes) ICC 1:500-1:5000 (see notes) |
| Formulation | Lyophilized hybridoma cell culture media with sodium azide |
| Storage Instruction | Spin the vial briefly before opening it. Reconstitute in 250 uL sterile-filtered, room temperature, ultrapure water. Let the vial rehydrate for 5 minutes. Mix gently with a pipette tip to help the redissolution of the material. Centrifuge to remove a |
Target Information
| Immunogen | Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae). |
| Immunogen Sequence | Yeast |
| Specificity | The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on Western blotting and shows a clear and strong punctate staining of yeast nuclei. It can |
Additional Info
| Background | Our Anti-rRNA 2 O-methyltransferase fibrillarin (Nop1p) mouse monoclonal primary antibody detects c. elegans, drosophila, human, other mammals (predicted) , rat, and yeast rRNA 2 O-methyltransferase fibrillarin (Nop1p) , and is igg. It is validated for use in ICC, WB. |
Information sourced from Uniprot.org