| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RRAGD (181-400) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:4000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RRAGD |
| Gene ID: |
58528 |
| Uniprot ID: |
RRAGD_HUMAN |
| Immunogen Region: |
181-400 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 181-400 of human RRAGD (NP_067067.1). |
| Immunogen Sequence: |
VDGLSDDHKIETQRDIHQRA NDDLADAGLEKIHLSFYLTS IYDHSIFEAFSKVVQKLIPQ LPTLENLLNIFISNSGIEKA FLFDVVSKIYIATDSTPVDM QTYELCCDMIDVVIDISCIY GLKEDGAGTPYDKESTAIIK LNNTTVLYLKEVTKFLALVC FVREESFERKGLIDYNFHCF RKAIHEVFEVRMKVVKSRKV QNRLQKKKRATPNGTPRVLL |
| Function | Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RagA/RRAGA or RagB/RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form: RagD/RRAGD is in its active form when GDP-bound RagD/RRAGD forms a complex with GTP-bound RagA/RRAGA (or RagB/RRAGB) and in an inactive form when GTP-bound RagD/RRAGD heterodimerizes with GDP-bound RagA/RRAGA (or RagB/RRAGB). In its active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the MTOR signaling cascade by amino acids. Also plays a central role in the non-canonical mTORC1 complex, which acts independently of RHEB and specifically mediates phosphorylation of MiT/TFE factors TFEB and TFE3: GDP-bound RagD/RRAGD mediates recruitment of MiT/TFE factors TFEB and TFE3. |
| Protein Name | Ras-Related Gtp-Binding Protein DRag DRagd |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-8943724Reactome: R-HSA-9639288 |
| Cellular Localisation | CytoplasmNucleusLysosome MembranePredominantly CytoplasmicRecruited To The Lysosome Surface By The Ragulator ComplexMay Shuttle Between The Cytoplasm And NucleusDepending On The Bound Nucleotide State Of Associated Rraga |
| Alternative Antibody Names | Anti-Ras-Related Gtp-Binding Protein D antibodyAnti-Rag D antibodyAnti-Ragd antibodyAnti-RRAGD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
