• Immunohistochemistry analysis of RRAGA in paraffin-embedded human placenta using RRAGA Rabbit polyclonal antibody (STJ117328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RRAGA Rabbit polyclonal antibody (STJ117328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RRAGA antibody (213-313) (STJ117328)

SKU:
STJ117328

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RRAGA (213-313) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRAGA
Gene ID: 10670
Uniprot ID: RRAGA_HUMAN
Immunogen Region: 213-313
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 213-313 of human RRAGA (NP_006561.1).
Immunogen Sequence: VISHYQCKEQRDVHRFEKIS NIIKQFKLSCSKLAASFQSM EVRNSNFAAFIDIFTSNTYV MVVMSDPSIPSAATLINIRN ARKHFEKLERVDGPKHSLLM R
Tissue Specificity Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
Post Translational Modifications Polybiquitinated via 'Lys-63'-linked polyubiquitination by RNF152 in response to amino acid starvation: polyubiquitination of the GDP-bound inactive form by RNF152 promotes RRAGA inactivation and interaction with the GATOR1 complex. This does not affect RRAGA degradation.
Function Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RagC/RRAGC or RagD/RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form: RagA/RRAGA is in its active form when GTP-bound RagA/RRAGA forms a complex with GDP-bound RagC/RRAGC (or RagD/RRAGD) and in an inactive form when GDP-bound RagA/RRAGA heterodimerizes with GTP-bound RagC/RRAGC (or RagD/RRAGD). In its GTP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Protein Name Ras-Related Gtp-Binding Protein A
Rag A
Raga
Adenovirus E3 14.7 Kda-Interacting Protein 1
Fip-1
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-8866654
Reactome: R-HSA-8943724
Reactome: R-HSA-9639288
Cellular Localisation Cytoplasm
Nucleus
Lysosome Membrane
Predominantly Cytoplasmic
Recruited To The Lysosome Surface By The Ragulator Complex
May Shuttle Between The Cytoplasm And Nucleus
Depending On The Bound Nucleotide State
Colocalizes In Vivo With Adenovirus E3-14
7k Mainly To The Cytoplasm Especially Near The Nuclear Membrane And In Discrete Foci On Or Near The Plasma Membrane
Alternative Antibody Names Anti-Ras-Related Gtp-Binding Protein A antibody
Anti-Rag A antibody
Anti-Raga antibody
Anti-Adenovirus E3 14.7 Kda-Interacting Protein 1 antibody
Anti-Fip-1 antibody
Anti-RRAGA antibody

Information sourced from Uniprot.org

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