• Immunofluorescence analysis of NIH-3T3 cells using RPS6KA1/RPS6KA2/RPS6KA3 Rabbit polyclonal antibody (STJ11103257) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RPS6KA1/RPS6KA2/RPS6KA3 in paraffin-embedded rat stomach using RPS6KA1/RPS6KA2/RPS6KA3 Rabbit polyclonal antibody (STJ11103257) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RPS6KA1/RPS6KA2/RPS6KA3 in paraffin-embedded mouse kidney using RPS6KA1/RPS6KA2/RPS6KA3 Rabbit polyclonal antibody (STJ11103257) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RPS6KA1/RPS6KA2/RPS6KA3 in paraffin-embedded human liver using RPS6KA1/RPS6KA2/RPS6KA3 Rabbit polyclonal antibody (STJ11103257) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RPS6KA1/RPS6KA2/RPS6KA3 Rabbit polyclonal antibody (STJ11103257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RPS6KA1/RPS6KA2/RPS6KA3 antibody (600-700) (STJ11103257)

SKU:
STJ11103257

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS6KA1/RPS6KA2/RPS6KA3 (600-700) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS6KA1
RPS6KA2
6195
6196
6197
KS6A1_HUMAN
KS6A2_HUMAN
KS6A3_HUMAN
Immunogen Region: 600-700
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human RPS6KA1/RPS6KA2/RPS6KA3 (NP_002944.2).
Immunogen Sequence: LGILLYTMLAGYTPFANGPS DTPEEILTRIGSGKFTLSGG NWNTVSETAKDLVSKMLHVD PHQRLTAKQVLQHPWVTQKD KLPQSQLSHQDLQLVKGAMA A
Tissue Specificity Q15418 - NA
Q15349 - Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
P51812 - Expressed in many tissues, highest levels in skeletal muscle.
Q15418 - NA
Q15349 - Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
P51812 - Expressed in many tissues, highest levels in skeletal muscle.
Alternative Antibody Names Anti-Ribosomal Protein S6 Kinase Alpha-1 antibody
Anti-S6k-Alpha-1 antibody
Anti-90 Kda Ribosomal Protein S6 Kinase 1 antibody
Anti-P90-Rsk 1 antibody
Anti-P90rsk1 antibody
Anti-P90s6k antibody
Anti-Map Kinase-Activated Protein Kinase 1a antibody
Anti-Mapk-Activated Protein Kinase 1a antibody
Anti-Mapkap Kinase 1a antibody
Anti-Mapkapk-1a antibody
Anti-Ribosomal S6 Kinase 1 antibody
Anti-Rsk-1 antibody
Anti-RPS6KA1 antibody
Anti-MAPKAPK1A antibody
Anti-RSK1 antibody
Anti-Ribosomal Protein S6 Kinase Alpha-2 antibody
Anti-S6k-Alpha-2 antibody
Anti-90 Kda Ribosomal Protein S6 Kinase 2 antibody
Anti-P90-Rsk 2 antibody
Anti-P90rsk2 antibody
Anti-Map Kinase-Activated Protein Kinase 1c antibody
Anti-Mapk-Activated Protein Kinase 1c antibody
Anti-Mapkap Kinase 1c antibody
Anti-Mapkapk-1c antibody
Anti-Ribosomal S6 Kinase 3 antibody
Anti-Rsk-3 antibody
Anti-Pp90rsk3 antibody
Anti-RPS6KA2 antibody
Anti-MAPKAPK1C antibody
Anti-RSK3 antibody
Anti-Ribosomal Protein S6 Kinase Alpha-3 antibody
Anti-S6k-Alpha-3 antibody
Anti-90 Kda Ribosomal Protein S6 Kinase 3 antibody
Anti-P90-Rsk 3 antibody
Anti-P90rsk3 antibody
Anti-Insulin-Stimulated Protein Kinase 1 antibody
Anti-Ispk-1 antibody
Anti-Map Kinase-Activated Protein Kinase 1b antibody
Anti-Mapk-Activated Protein Kinase 1b antibody
Anti-Mapkap Kinase 1b antibody
Anti-Mapkapk-1b antibody
Anti-Ribosomal S6 Kinase 2 antibody
Anti-Rsk-2 antibody
Anti-Pp90rsk2 antibody
Anti-RPS6KA3 antibody
Anti-ISPK1 antibody
Anti-MAPKAPK1B antibody
Anti-RSK2 antibody
Anti-Ribosomal Protein S6 Kinase Alpha-1 antibody
Anti-S6k-Alpha-1 antibody
Anti-90 Kda Ribosomal Protein S6 Kinase 1 antibody
Anti-P90-Rsk 1 antibody
Anti-P90rsk1 antibody
Anti-P90s6k antibody
Anti-Map Kinase-Activated Protein Kinase 1a antibody

Information sourced from Uniprot.org

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