• Immunofluorescence analysis of NIH/3T3 cells using S6 Ribosomal Protein (RPS6) Rabbit polyclonal antibody (STJ27852) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon using S6 Ribosomal Protein (RPS6) Rabbit polyclonal antibody (STJ27852) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using S6 Ribosomal Protein (RPS6) antibody (STJ27852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS6 antibody (1-130) (STJ27852)

SKU:
STJ27852

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS6 (1-130) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity purification
Gene Symbol: RPS6
Gene ID: 6194
Uniprot ID: RS6_HUMAN
Immunogen Region: 1-130
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-130 of human RPS6 (NP_001001.2).
Immunogen Sequence: MKLNISFPATGCQKLIEVDD ERKLRTFYEKRMATEVAADA LGEEWKGYVVRISGGNDKQG FPMKQGVLTHGRVRLLLSKG HSCYRPRRTGERKRKSVRGC IVDANLSVLNLVIVKKGEKD IPGLTDTTVP
Post Translational Modifications Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3.phosphorylation at these sites facilitates the assembly of the pre-initiation complex. Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8. Mono-ADP-ribosylation at Glu-35 by PARP16 inhibits polysome assembly and mRNA loading, thereby inhibiting protein translation.
Function Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Small Ribosomal Subunit Protein Es6
40s Ribosomal Protein S6
Phosphoprotein Np33
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-166208
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9725371
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Es6 antibody
Anti-40s Ribosomal Protein S6 antibody
Anti-Phosphoprotein Np33 antibody
Anti-RPS6 antibody
Anti-OK antibody
Anti-SW-cl.2 antibody

Information sourced from Uniprot.org

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