• Western blot analysis of extracts of various cell lines, using RPS27 antibody (STJ28812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS27 antibody (1-84) (STJ28812)

SKU:
STJ28812

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS27 (1-84) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS27
Gene ID: 6232
Uniprot ID: RS27_HUMAN
Immunogen Region: 1-84
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human RPS27 (NP_001021.1).
Immunogen Sequence: MPLAKDLLHPSPEEEKRKHK KKRLVQSPNSYFMDVKCPGC YKITTVFSHAQTVVLCVGCS TVLCQPTGGKARLTEGCSFR RKQH
Tissue Specificity Expressed in a wide variety of actively proliferating cells and tumor tissues.
Function Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for proper rRNA processing and maturation of 18S rRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Small Ribosomal Subunit Protein Es27
40s Ribosomal Protein S27
Metallopan-Stimulin 1
Mps-1
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-6791226
Reactome: R-HSA-68877
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9648025
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Es27 antibody
Anti-40s Ribosomal Protein S27 antibody
Anti-Metallopan-Stimulin 1 antibody
Anti-Mps-1 antibody
Anti-RPS27 antibody
Anti-MPS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance