| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RPS2 (1-293) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RPS2 |
| Gene ID: |
6187 |
| Uniprot ID: |
RS2_HUMAN |
| Immunogen Region: |
1-293 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human RPS2 (NP_002943.2). |
| Immunogen Sequence: |
MADDAGAAGGPGGPGGPGMG NRGGFRGGFGSGIRGRGRGR GRGRGRGRGARGGKAEDKEW MPVTKLGRLVKDMKIKSLEE IYLFSLPIKESEIIDFFLGA SLKDEVLKIMPVQKQTRAGQ RTRFKAFVAIGDYNGHVGLG VKCSKEVATAIRGAIILAKL SIVPVRRGYWGNKIGKPHTV PCKVTGRCGSVLVRLIPAPR GTGIVSAPVPKKLLMMAGID DCYTSARGCTATLGNFAKA |
| Post Translational Modifications | Citrullinated by PADI4 in the Arg/Gly-rich region. Asymmetric arginine dimethylation by PRMT3 occurs at multiple sites in the Arg/Gly-rich region. Monoubiquitinated at Lys-54 and Lys-58 by RNF10 when a ribosome has stalled during translation, leading to its degradation by the proteasome. Deubiquitinated at Lys-54 and Lys-58 by USP10, preventing degradation by the proteasome and promoting 40S ribosome subunit recycling following ribosome dissociation. |
| Function | Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Plays a role in the assembly and function of the 40S ribosomal subunit. Mutations in this protein affects the control of translational fidelity. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. |
| Protein Name | Small Ribosomal Subunit Protein Us540s Ribosomal Protein S240s Ribosomal Protein S4Protein Llrep3 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-1799339Reactome: R-HSA-192823Reactome: R-HSA-2408557Reactome: R-HSA-3214858Reactome: R-HSA-6790901Reactome: R-HSA-6791226Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-8876725Reactome: R-HSA-9010553Reactome: R-HSA-9633012Reactome: R-HSA-9735869Reactome: R-HSA-9754678Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmNucleusNucleolusProbably Localized To Nucleolus And Cytoplasm In Complex With Znf277 |
| Alternative Antibody Names | Anti-Small Ribosomal Subunit Protein Us5 antibodyAnti-40s Ribosomal Protein S2 antibodyAnti-40s Ribosomal Protein S4 antibodyAnti-Protein Llrep3 antibodyAnti-RPS2 antibodyAnti-RPS4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit polyclonal antibody (STJ11100043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89115/357567/STJ11100043_1__91960.1713121694.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit polyclonal antibody (STJ11100043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
