| Host: | Rabbit |
| Applications: | WB/IHC/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-RPS10 (1-165) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:1000IHC-P 1:20-1:200IF/ICC 1:20-1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPS10 |
| Gene ID: | 6204 |
| Uniprot ID: | RS10_HUMAN |
| Immunogen Region: | 1-165 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPS10 (NP_001190174.1). |
| Immunogen Sequence: | MLMPKKNRIAIYELLFKEGV MVAKKDVHMPKHPELADKNV PNLHVMKAMQSLKSRGYVKE QFAWRHFYWYLTNEGIQYLR DYLHLPPEIVPATLRRSRPE TGRPRPKGLEGERPARLTRG EADRDTYRRSAVPPGADKKA EAGAGSATEFQFRGGFGRGR GQPPQ |
| Post Translational Modifications | Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation. Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide. Deubiquitinated by OTUD3 and USP21, antagonizing ZNF598 activity. Deubiquitinated by OTUD1, antagonizing ZNF598 activity and stimulating formation of polysomes: deubiquitination by OTUD1 promotes stability and translation of a subset mRNAs with a high abundance of rare codons can limit the translation rate. Deubiquitinated by USP10. |
| Function | Component of the 40S ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. |
| Protein Name | Small Ribosomal Subunit Protein Es1040s Ribosomal Protein S10 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-1799339Reactome: R-HSA-192823Reactome: R-HSA-2408557Reactome: R-HSA-6791226Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-9633012Reactome: R-HSA-9735869Reactome: R-HSA-9754678Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmNucleusNucleolusLocalized In The Granular Component (Gc) Region Of The NucleolusMethylation Is Required For Its Localization In The Gc RegionColocalizes With Nps1 In The Gc Region Of The Nucleolus |
| Alternative Antibody Names | Anti-Small Ribosomal Subunit Protein Es10 antibodyAnti-40s Ribosomal Protein S10 antibodyAnti-RPS10 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
