• Immunofluorescence analysis of U-2 OS cells using RPP38 Rabbit polyclonal antibody (STJ119300) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RPP38 in paraffin-embedded Human lung cancer using RPP38 Rabbit polyclonal antibody (STJ119300) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RPP38 Rabbit polyclonal antibody (STJ119300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPP38 antibody (20-110) (STJ119300)

SKU:
STJ119300

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPP38 (20-110) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPP38
Gene ID: 10557
Uniprot ID: RPP38_HUMAN
Immunogen Region: 20-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human RPP38 (NP_892117.1).
Immunogen Sequence: VVKTSLNNPYIIRWSALESE DMHFILQTLEDRLKAIGLQK IEDKKKKNKTPFLKKESREK CSIAVDISENLKEKKTDAKQ QVSGWTPAHVR
Function Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.
Protein Name Ribonuclease P Protein Subunit P38
Rnasep Protein P38
Database Links Reactome: R-HSA-6784531
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Ribonuclease P Protein Subunit P38 antibody
Anti-Rnasep Protein P38 antibody
Anti-RPP38 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance