Western blot analysis of various lysates using RPP30 Rabbit pAb (STJ110777) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-RPP30 antibody (1-268) (STJ110777)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110777
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General Information

Short DescriptionRabbit polyclonal RPP30 (1-268) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:100-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRPP30
Gene ID10556
Uniprot IDRPP30_HUMAN
Immunogen Region1-268
Immunogen SequenceMAVFADLDLRAGSDLKALRG LVETAAHLGYSVVAINHIVD FKEKKQEIEKPVAVSELFTT LPIVQGKSRPIKILTRLTII VSDPSHCNVLRATSSRARLY DVVAVFPKTEKLFHIACTHL DVDLVCITVTEKLPFYFKRP PINVAIDRGLAFELVYSPAI KDSTMRRYTISSALNLMQIC KGKNVIISSAAERPLEIRGP YDVANLGLLFGLSESDAKAA VSTNCRAALLHGETRKTAF
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human RPP30 (NP_006404.1).

Additional Info

Function Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.
Protein Name Ribonuclease P Protein Subunit P30
Rnasep Protein P30
Rnase P Subunit 2
Database Links Reactome: R-HSA-6784531
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Ribonuclease P Protein Subunit P30 antibody
Anti-Rnasep Protein P30 antibody
Anti-Rnase P Subunit 2 antibody
Anti-RPP30 antibody
Anti-RNASEP2 antibody

Information sourced from Uniprot.org

Citations

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