Anti-RPN1 antibody (20-280) (STJ114371)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RPN1 (20-280) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RPN1 |
| Gene ID | 6184 |
| Uniprot ID | RPN1_HUMAN |
| Immunogen Region | 20-280 |
| Immunogen Sequence | APGSASSEAPPLINEDVKRT VDLSSHLAKVTAEVVLAHLG GGSTSRATSFLLALEPELEA RLAHLGVQVKGEDEEENNLE VRETKIKGKSGRFFTVKLPV ALDPGAKISVIVETVYTHVL HPYPTQITQSEKQFVVFEGN HYFYSPYPTKTQTMRVKLAS RNVESYTKLGNPTRSEDLLD YGPFRDVPAYSQDTFKVHYE NNSPFLTITSMTRVIEVSHW GNIAVEENVDLKHTGAVLK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1). |
Additional Info
| Tissue Specificity | Expressed in all tissues tested. |
| Post Translational Modifications | Ubiquitinated by the ECS(ASB11) complex. Ubiquitinated by RNF128, leading to degradation in a proteasome/lysosome-dependent manner. Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets. |
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
| Protein Name | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda SubunitRibophorin IRpn-IRibophorin-1 |
| Database Links | Reactome: R-HSA-1799339Reactome: R-HSA-446203Reactome: R-HSA-9694548 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1 antibodyAnti-Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda Subunit antibodyAnti-Ribophorin I antibodyAnti-Rpn-I antibodyAnti-Ribophorin-1 antibodyAnti-RPN1 antibody |
Information sourced from Uniprot.org