• Immunofluorescence analysis of U-2 OS cells using RPL32 Rabbit polyclonal antibody (STJ114970) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RPL32 Rabbit polyclonal antibody (STJ114970) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RPL32 in paraffin-embedded mouse spinal cord using RPL32 Rabbit polyclonal antibody (STJ114970) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RPL32 in paraffin-embedded human lung cancer using RPL32 Rabbit polyclonal antibody (STJ114970) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RPL32 in paraffin-embedded rat brain using RPL32 Rabbit polyclonal antibody (STJ114970) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RPL32 Rabbit polyclonal antibody (STJ114970) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RPL32 antibody (1-135) (STJ114970)

SKU:
STJ114970

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL32 (1-135) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL32
Gene ID: 6161
Uniprot ID: RL32_HUMAN
Immunogen Region: 1-135
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1).
Immunogen Sequence: MAALRPLVKPKIVKKRTKKF IRHQSDRYVKIKRNWRKPRG IDNRVRRRFKGQILMPNIGY GSNKKTKHMLPSGFRKFLVH NVKELEVLLMCNKSYCAEIA HNVSSKNRKAIVERAAQLAI RVTNPNARLRSEENE
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein El32
60s Ribosomal Protein L32
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein El32 antibody
Anti-60s Ribosomal Protein L32 antibody
Anti-RPL32 antibody
Anti-PP9932 antibody

Information sourced from Uniprot.org

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