• Confocal immunofluorescence analysis of HeLa cells using RPL27 Rabbit polyclonal antibody (STJ115011) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using RPL27 Rabbit polyclonal antibody (STJ115011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using RPL27 Rabbit polyclonal antibody (STJ115011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using RPL27 Rabbit polyclonal antibody (STJ115011) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RPL27 Rabbit polyclonal antibody (STJ115011) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RPL27 antibody (1-136) (STJ115011)

SKU:
STJ115011

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL27 (1-136) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL27
Gene ID: 6155
Uniprot ID: RL27_HUMAN
Immunogen Region: 1-136
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human RPL27 (NP_000979.1).
Immunogen Sequence: MGKFMKPGKVVLVLAGRYSG RKAVIVKNIDDGTSDRPYSH ALVAGIDRYPRKVTAAMGKK KIAKRSKIKSFVKVYNYNHL MPTRYSVDIPLDKTVVNKDV FRDPALKRKARREAKVKFEE RYKTGKNKWFFQKLRF
Function Component of the large ribosomal subunit. Required for proper rRNA processing and maturation of 28S and 5.8S rRNAs.
Protein Name Large Ribosomal Subunit Protein El27
60s Ribosomal Protein L27
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Cytosol
Rough Endoplasmic Reticulum
Detected On Cytosolic Polysomes
Detected In Ribosomes That Are Associated With The Rough Endoplasmic Reticulum
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein El27 antibody
Anti-60s Ribosomal Protein L27 antibody
Anti-RPL27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance