• Confocal immunofluorescence analysis of HeLa cells using RPL23A antibody (STJ25395) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using RPL23A antibody (STJ25395) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-431 cells using RPL23A antibody (STJ25395) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using RPL23A Rabbit polyclonal antibody (STJ25395) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using RPL23A Rabbit polyclonal antibody (STJ25395) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal using RPL23A Rabbit polyclonal antibody (STJ25395) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using RPL23A Rabbit polyclonal antibody (STJ25395) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPL23A antibody (STJ25395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RPL23A antibody (1-156) (STJ25395)

SKU:
STJ25395

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL23A (1-156) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL23A
Gene ID: 6147
Uniprot ID: RL23A_HUMAN
Immunogen Region: 1-156
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPL23A (NP_000975.2).
Immunogen Sequence: MAPKAKKEAPAPPKAEAKAK ALKAKKAVLKGVHSHKKKKI RTSPTFRRPKTLRLRRQPKY PRKSAPRRNKLDHYAIIKFP LTTESAMKKIEDNNTLVFIV DVKANKHQIKQAVKKLYDID VAKVNTLIRPDGEKKAYVRL APDYDALDVANKIGII
Post Translational Modifications N-terminus is methylated by METTL11A/NTM1. Citrullinated by PADI4.
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds a specific region on the 26S rRNA. May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination.
Protein Name Large Ribosomal Subunit Protein Ul23
60s Ribosomal Protein L23a
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Although Rpl23a Is Functional Within The Cytoplasm
The Assembly Of Ribosomal Subunits Occurs In The Nucleus
Rpl23a Nuclear Import Is Mediated By Ipo5/Ranbp5
Ipo7/Ranbp7
Kpnb1/Importin-Beta Or Tpno1/Trn
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul23 antibody
Anti-60s Ribosomal Protein L23a antibody
Anti-RPL23A antibody

Information sourced from Uniprot.org

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