• Confocal immunofluorescence analysis of HeLa cells using RPL23 Polyclonal Antibody (STJ111217) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using RPL23 antibody (STJ111217) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using RPL23 antibody (STJ111217) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RPL23 Rabbit polyclonal antibody (STJ111217) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using RPL23 Rabbit polyclonal antibody (STJ111217) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPL23 antibody (STJ111217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-RPL23 antibody (1-140) (STJ111217)

SKU:
STJ111217

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL23 (1-140) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:4000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL23
Gene ID: 9349
Uniprot ID: RL23_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human RPL23 (NP_000969.1).
Immunogen Sequence: MSKRGRGGSSGAKFRISLGL PVGAVINCADNTGAKNLYII SVKGIKGRLNRLPAAGVGDM VMATVKKGKPELRKKVHPAV VIRQRKSYRRKDGVFLYFED NAGVIVNNKGEMKGSAITGP VAKECADLWPRIASNAGSIA
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein Ul14
60s Ribosomal Protein L17
60s Ribosomal Protein L23
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul14 antibody
Anti-60s Ribosomal Protein L17 antibody
Anti-60s Ribosomal Protein L23 antibody
Anti-RPL23 antibody

Information sourced from Uniprot.org

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